Protocols
Snail Protocols
Unauthorized HOWTOs
Detailed protocols from Sample to Sequence
These are taken from the Barber lab where I used to work.
- DNA Extraction via quick and cheap chelex beads
- PCR using standard "hot start" conditions
- Agarose gel electrophoresis for visualizing PCR fragments
- SAP/Exo cleanup to get PCR product ready for sequencing
- Cycle Sequencing to get DNA sequence data